Gallery Links
Users Online
· Guests Online: 11

· Members Online: 0

· Total Members: 4,334
· Newest Member: BUCAILLE
Forum Threads
Theme Switcher
Switch to:
Last Seen Users
· Barry00:07:04
· atylotus00:16:50
· Zeegers00:18:11
· ebbek00:21:36
· binturong00:24:10
· Tony Irwin00:26:46
· Cranefly00:47:15
· libor01:01:10
· Gansucha01:13:52
· John Carr01:31:23
Latest Photo Additions
View Thread
Diptera.info :: General Diptera forums :: Methodology
Who is here? 1 guest(s)
 Print Thread
DNA extraction from samples
RobertOBaggio
#1 Print Post
Posted on 09-04-2014 19:14
Member

Location:
Posts: 43
Joined: 31.01.14

Hello folks,
I am hoping to extract DNA from samples collected from Malaise traps. Whilst I have been warned about using 70% IMS in my collection bottles due to DNA denaturation, I was hoping that someone might be able to comment on one proposed way around of having to use ethanol in my collecting heads:

If the insects are trapped in IMS (70%) and transferred weekly to ethanol, then the DNA should not degrade very much. I have read that IMS degradation occurs over periods of weeks rather than days.

Cheers.
 
jonas
#2 Print Post
Posted on 10-04-2014 14:03
Member

Location:
Posts: 334
Joined: 06.05.07

Robert,
70% ethanol for DNA extractions, from malaisetraps works perfect. No worries.

In a barcoding project on Empidoidea we got a very high success rate for COI. All samples were malaisetrap samples collected directly EtOh, stored for two weeks at 20° (in the dark!), and then identified and each specimen put in a separate vial in 96% EtOh.

I have no experience with IMS - but why not collect directly in EtOh?

Please, for details check our publication: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890682/
Cheers
 
RobertOBaggio
#3 Print Post
Posted on 10-04-2014 14:06
Member

Location:
Posts: 43
Joined: 31.01.14

Thanks Jonas,

The issue with EtOh is cost. I will be running 24 Malaise traps for 5 months (weekly collections). IMS is around 5 times cheaper than EtOH. I will defo read your paper. Many thanks for the reply. I hope to do some barcoding myself so fingers crossed!


 
ChrisR
#4 Print Post
Posted on 12-04-2014 12:08
User Avatar

Administrator

Location: Reading, England
Posts: 7694
Joined: 12.07.04

Where will you be collecting? Remember to keep any tachinids Grin
Manager of the UK Species Inventory in the Angela Marmont Centre for UK Biodiversity at the Natural History Museum, London.
 
http://tachinidae.org.uk
libor
#5 Print Post
Posted on 12-04-2014 17:21
Member

Location: western Bohemia
Posts: 1092
Joined: 30.05.09

Roberto, I would highly appreciate Anisopodidae, Rhagionidae, and Athericidae. Is this material from your traps free?
Thanks
Libor
 
Jump to Forum:
Similar Threads
Thread Forum Replies Last Post
Long distance transport of Malaise-trap samples Methodology 1 18-11-2018 12:57
Extraction of the mouthparts of an Hymenoptera species Other insects, spiders, etc. 1 25-05-2017 17:37
Date and time
07 December 2019 20:28
Login
Username

Password



Not a member yet?
Click here to register.

Forgotten your password?
Request a new one here.
Temporary email?
Due to fact this site has functionality making use of your email address, any registration using a temporary email address will be rejected.

Paul
Donate
Please, help to make
Diptera.info
possible and enable
further improvements!
Latest Articles
Syrph the Net
Those who want to have access to the Syrph the Net database need to sign the
License Agreement -
Click to Download


Public files of Syrph the Net can be downloaded HERE

Last updated: 25.08.2011
Shoutbox
You must login to post a message.

04.12.19 10:23
Thx! TumbsUp

04.12.19 09:33
There are some internal issues withy their servers and they are working on it.

02.12.19 08:42
Anyone knows what happened with the Catalog of Fossil Diptera at the bishopmuseum.org webpage? Cannot access it Frown

01.11.19 22:46
Hi! may I ask, why the soldierfly banner (https://www.dipte
ra.info/images/sol
dierfly-banner.png
) is so big (4790x870 Pixels, 1, 4 M Cool? It always lasts minutes to load on a slow connection and costs a

18.09.19 20:06
Jewell699, you should post it in the forum, not submit it for the gallery.

15.09.19 20:41
Jewelm699 - did you upload it to a forum (which one?) or the gallery? I can't find it.

11.09.19 13:15
I’m hoping someone can identify the phoridae maggot or pupae I just uploaded.

28.08.19 14:29
Rafael p is legs and f1 is fore femur

26.08.19 17:13
If you experienced a very sluggish site recently, it may have been because someone tried to upload a maliciuous script by force. It appears to have failed. The visitor was blocked further access.

25.07.19 15:13
@Paul Beuk Thanks mate!! Best wishes!

Render time: 2.95 seconds | 142,953,699 unique visits