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Diptera.info :: Identification queries :: Diptera (adults)
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Small acalyptrate fly - Diastata flavicosta (or fuscula)
djo
#1 Print Post
Posted on 16-08-2023 19:37
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Location: Edinburgh, UK
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Aspirated from a fallen deciduous tree trunk in Sussex, UK, August 2023. The scale intervals are 0.5mm
djo attached the following image:


[275.96Kb]
Edited by djo on 17-08-2023 12:04
 
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djo
#2 Print Post
Posted on 16-08-2023 19:38
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Location: Edinburgh, UK
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Aspirated from a fallen deciduous tree trunk in Sussex, UK, August 2023. The scale intervals are 0.5mm
djo attached the following image:


[255.75Kb]
 
obbard.bio.ed.ac.uk/
Tony Irwin
#3 Print Post
Posted on 17-08-2023 08:03
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Diastata?
Tony
----------
Tony Irwin
 
djo
#4 Print Post
Posted on 17-08-2023 09:22
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Hmmm .... someone else (iNat) suggested Trixoscelis (Heleomyzidae). The spikes on the costa look right for that, but the setae on the mesonotum look wrong.

Diastata (cf costata?) looks like a good idea!
Edited by djo on 17-08-2023 09:26
 
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Jan Maca
#5 Print Post
Posted on 17-08-2023 10:11
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Diastata, looks like D. flavicosta but a rather detailed study (arista, genitalia) is needed to distinguish it from D. fuscula. I suggest to send the specimen to Peter Chandler.
 
djo
#6 Print Post
Posted on 17-08-2023 14:14
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Location: Edinburgh, UK
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Hmmm, looks like fuscula but not flavicosta is in the UK list.

Unfortunately, I want to sequence the genome - which is not compatible with ding anything else to the specimen!
 
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Tony Irwin
#7 Print Post
Posted on 17-08-2023 20:27
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Darren - you shouldn't need the whole specimen for DNA investigation. Remove the posterior half of the abdomen - this has all you need to identify the specimen morphologically (male genitalia). And the remainder of the specimen will be plenty to extract your DNA samples.
Tony
----------
Tony Irwin
 
djo
#8 Print Post
Posted on 18-08-2023 09:33
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Location: Edinburgh, UK
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Hi!

Unfortunately genome sequencing requires a lot more DNA than for PCR/Sanger for COI etc (which need <1ng DNA). Minimum for Novaseq/short-read genome might be ~20ng at ~2ng/ul, and in fact we often struggle to get enough at sufficient concentration from single Drosophila.

But this would actually be going for ONT long read, which really would benefit from the whole fly. For comparison, Darwin tree of life ask for 4 specimens when doing stuff this small (Hifi,HiC,RNA, and spare!)

It's part of a Drosophilidae project, but the world is desperately short of suitable outgroup genomes (i.e. Ephydroidea) and so my collaborator is searching for (fresh, -20C in Ethanol) specimens to sequence!

The species really don't matter, so long as they sit between Opomyzoidea/Carnoidea and Drosophilidae!

I'll check with my collaborator, and see what he thinks.

Thanks!

D
 
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Some flies preserved in ethanol and then pinned often get the eyes sunken, how can this be avoided? Best answer: I usually keep alcohol-collected material in alcohol

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