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Diptera.info :: Identification queries :: Diptera (eggs, larvae, pupae)
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Tipula obscuriventris hosting endoparasitoids
solito de solis
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Posted on 22-09-2015 08:46
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Location: Liege, Belgium
Posts: 404
Joined: 05.08.13

The bigger Tipula obscuriventris larvae from the last video
has been cut and opened to sample a piece of skin and verify the setae position and distribution on the abdominal segments

And inside of the body there were 7 living larvae hung to the tracheal trunks
at the level of segments VII, VIII

These larvae have been observed anf we can see some pictures about a few features of its
We suppose that they could be Tachinids larvae (Siphona ?) asTachinids are well-known to have this habit of endoparasiting Tipulids

Here are the pictures. Larvae are 3 mm length, are white to transparent
and fixed chitinously on the tracheal trunks of Tipula

Please, we'll be very happy to make other pictures on these larvae if it could help to determinate better these organisms
Just tell me what could be observed and photographied to reach this goal

rodredi.free.fr/endotipu/endotipu01.jpg

rodredi.free.fr/endotipu/endotipu02.jpg

prothoracic stigmate
rodredi.free.fr/endotipu/endotipu03.JPG

rodredi.free.fr/endotipu/endotipu04.JPG

rodredi.free.fr/endotipu/endotipu05.jpg

rodredi.free.fr/endotipu/endotipu06.jpg

dorsal view of cephalic skeleton
rodredi.free.fr/endotipu/endotipu07.jpg

spicules all over the skin
rodredi.free.fr/endotipu/endotipu08.jpg


rodredi.free.fr/endotipu/endotipu09.jpg




YouTube Video



merci for the attention
saludos to diptera fellows
SDS
Edited by solito de solis on 22-09-2015 09:35
 
solito de solis
#2 Print Post
Posted on 22-09-2015 14:46
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Location: Liege, Belgium
Posts: 404
Joined: 05.08.13

I opened a new Tipula obscuriventris larva standing in the same sample


rodredi.free.fr/endotipu/endotipua1.jpg



This larvae was shorter (about 40 mm length)
and I found another Diptera lava attached to tracheal trunks of Tipulida at the level of Abdomen Semgent II

This larvae seemed to me to be wrapped by some organic "bag" well ornamented by tracheas

rodredi.free.fr/endotipu/endotipua8.jpg



the wrapping bag is a little bit yellow colored
with a chitinous black point where the Tachinid (?) larva sticks to the tracheal trunk of Tipula

On the transparent segments of the endoparasitoid can be seen rows of dark spicules
This larvae seemed to me to be older than the others larvae founded in the first observation

Same cephalic skeleton and a little piece of dark material found in each of all those larvae:
is it the tool used to puncture the Tipula trachea ?

merci pour l'attention

the Tipula larvae opened and the (Tachinid) endoparasit visible



rodredi.free.fr/endotipu/endotipua2.jpg



rodredi.free.fr/endotipu/endotipua3.jpg

The larva parasitoid is wrapped in a organic cuticule

rodredi.free.fr/endotipu/endotipua4.jpg

aspect of the spicules raws on the abdominal segments

rodredi.free.fr/endotipu/endotipua5.jpg

rodredi.free.fr/endotipu/endotipua6.jpg



rodredi.free.fr/endotipu/endotipua7.jpg






POsterior stigmata

rodredi.free.fr/endotipu/endotipua9.jpg
 
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