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Diptera.info :: General Diptera forums :: Methodology
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Slide mount question for chironomids
kschnei1000
#1 Print Post
Posted on 16-08-2021 18:20
Member

Location: California, USA
Posts: 58
Joined: 26.01.21

Hi all,

I am trying for the first time to make permanent slide mounts of chironomids, using the protocol suggested in "Chironomidae of the Holarctic Region, parts 3 adult males". Although I started with a modestly-sized midge (about 5 mm), I'm finding it very difficult to see how I can fit all the dissected parts under five small coverslips on a single microscope slide, as suggested by that reference. I am using standard 3 x 1 inch slides (sorry, no metric on the box I have) and 12-mm coverslips from Ted Pella. The first two cover slips are already taking up a bit too much space and the balsam is running all over the place. It seems like it will be quite difficult to use cover slips that are big enough to cover the dissected parts, but small enough to fit five on a standard glass slide? Do folks have suggestions? Should I be using extra large glass slides or using two slides per fly?

Thanks!

Ken Schneider
San Francisco, CA, USA
 
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23.06.25 18:10
If you have some spare money, there is a copy (together with keys to pupae and larvae) for sale by Hermann L. Strack, Loguivy Plougras, France

23.06.25 11:18
Appreciate it, Tony Irwin! I got the hint to use the key next to Langton and Pinder key for females of Chironomidae. So no specific queries, except the keys... I will keep this on my list and hope th

19.06.25 15:33
I have the hard copy book, if you have any specific queries, but I'm not scanning the 500+ pages!

02.06.25 18:26
Anyone has "Chironomidae of the Holarctic region. Keys and diagnoses. Part 3. Adult Males Entomologica Scandinavica Supplement 34"? smolwaarneming@gma
il.com

28.05.25 20:57
I have Russian Coenosia. nikita6510@ya.ru

28.05.25 12:25
Is someone able to share with me "A key to the Russian species of the genus Coenosia"?

08.05.25 18:22
I have

03.05.25 08:35
Does someone has a scan of Nartshuk E.P. 2003. Key to families of Diptera (Insecta) of the fauna of Russian and adjacent countries. Proceedings of the Zoological Institute Vol. 294: 1-252 for me?

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