Thread subject: Diptera.info :: Storing flies in alcohol

Posted by Tony T on 31-07-2008 13:52
#82

jorgemotalmeida wrote:
Ralph Sipple wrote:
Hi together,

sorry for necroing this thread.

jorgemotalmeida wrote: ..."NEVER use formaldehyde because it alters the structures of the specimens"...


Regards
Ralph


Maybe in infinitesimal quantities it doesn't harm the specimens. But if you only use Formaldehyde it is sure that it will damage the structures of the specimens.



"The Preservation of Natural History Specimens. I. Invertebrates. R. Wagstaff and J. H. Fidler. 1961."
I have found this to be a most authoratative text.
pp. 171-172: "Formalin - this is the most useful preservative. It is slightly acid and may damage specimens containing calcareous matter. it may be neutralized by being shaken with powdered chalk (5 gm/litre) and then filtered. Borax may also be used for the same purpose.
If formalin is neutralized the colours of specimens are generally better preserved than when in alcohol.
Use at 3-10% for storage.
As a preservative formalin is to be recommended for the more delicate soft-bodied animals as if used in the correct proportions it does not cause shrinkage"

I (TT) used to use formalin to 'fix' animal tissue prior to section cutting on a microtome. Tissue and cells were perfectly preserved; not sure what Jorge means by "it will damage the structures of the specimens"

Edited by Tony T on 31-07-2008 13:55