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HI

I use a Canon 30D, Kenko extension tubes (full set), and a Sigma 105 mm EX lens. The flash is a Canon 430 EX. I use ISO 200 (better for macro, usually), and f/11 to f/16 with 1/250, sometimes I use 1/60 and flash off. Find out for Mcyteridae in search, you will find a photo with 1/60 s and without flash.
The EV depends of the light condition. Usually between +0 ev to +1 1/3 . it depends.