Thread subject: Diptera.info :: DNA extraction from samples

Posted by jonas on 10-04-2014 13:03
#2

Robert,
70% ethanol for DNA extractions, from malaisetraps works perfect. No worries.

In a barcoding project on Empidoidea we got a very high success rate for COI. All samples were malaisetrap samples collected directly EtOh, stored for two weeks at 20° (in the dark!), and then identified and each specimen put in a separate vial in 96% EtOh.

I have no experience with IMS - but why not collect directly in EtOh?

Please, for details check our publication: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890682/
Cheers