Thread subject: Diptera.info :: DNA extraction from samples

Posted by RobertOBaggio on 09-04-2014 18:14
#1

Hello folks,
I am hoping to extract DNA from samples collected from Malaise traps. Whilst I have been warned about using 70% IMS in my collection bottles due to DNA denaturation, I was hoping that someone might be able to comment on one proposed way around of having to use ethanol in my collecting heads:

If the insects are trapped in IMS (70%) and transferred weekly to ethanol, then the DNA should not degrade very much. I have read that IMS degradation occurs over periods of weeks rather than days.

Cheers.