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Thanks for the compliment. This image is 43K, you should see the original 24MB image:D.
This is a stacked image of about 15 images, each at a slightly different focus. I stack them using Helicon Focus. I use a Nikon digital camera, a manual focus 105mm Nikon Micro lens coupled to a 52.5mm extension tube (PN11). For very small flies I use 2 PN11's + a 1.4x teleconverter.
For lighting, a single Nikon SB800 flash.
Pinned fly is surrounded by tube of white paper, flash aimed at ouside of this tube.