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Hey dipterists

from a stream freshwater sample collected and preserved
A strange pupa a little bit disymetrical in the posterior part, spindle shaped, the anterior tip very sharp
3 mm length litgh dark pattern on the skin

A larva inside correctly preserved after 5 years in formaline/ethanol extracted without difficulties

little head with short antennae and 2 spiracles on each side of the head














a strange button at the end of a fresher pupa last segment



spicules on the skin of the pupa




a unique pore on the top of the anterior part of the pupa










last abdominal segment


spiracles on each side of the head










thanks for the help

solito de solis

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Cecidomyidae. As far as I can tell there are no aquatic species.

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Merci Atylotus

You know that as we have to collect water samples in 8 places if a place in a stream
we need to move and shake aquatic plants, phragmites and bryophits we often find non-aquatic organisms from plant debris such as slugs, ladybugs, a.s.o.
I have been told that they are some CECIDOMYIIDA : PORRICONDYLINAE
living in such as aquatic biocoenosis... (torrential stream France SW)
(http://www.limnol...63p225.pdf )

Regarding the organism found in this pupa, could we ID better ?
Or we have to stop here by lack of features ?
SDS

MAYETIOLIA ?